Biosynthesis of poriferasterol in Ochromonas malhamensis: 13C NMR assignment of the isopropyl methyl groups of 2-methylpentan-3-ol

Abstract

2-Methylpentan-3-ol stereospecifically labelled with deuterium on one of the isopropyl methyl groups has been synthesized, thus allowing the unequivocal assignment of their ¹³C NMR resonances. ¹³C NMR assignments for these groups proposed earlier are shown to be incorrect. Involvement in the biosynthesis of the ²⁴β-ethylsterol poriferasterol in the crysophyte Ochromonas malhamensis is discussed.