Matrix surface modification by plasma polymerization for enzyme immobilization

Abstract

Polycarbonate (PC) membranes have been treated with dimethylamine (DMA), n-pentylamine (PA) or n-heptylamine (HA) in a glow-discharge apparatus. Amino group concentrations on plasma-polymerized PC membranes were then assayed by binding radiolabelled [1-¹⁴C]acetic anhydride to the membrane, followed by scintillation counting. For the different plasma-polymerized membranes different degrees of radiolabelled (¹²⁵I) glucose oxidase or rennet binding were observed and this was found to be directly related to the surface amino-group concentrations for the appropriate membrane.