Incubation of [18O2]valine with purified ACV synthetase from Cephalosporium acremonium gave exclusive incorporation of a single 18O label into δ-(L-α-aminoadipoyl)-L-cysteinyl-D-valine (ACV), consistent with the formation of a covalent valinoyl-ACV synthetase complex.
J. E. Baldwin, R. A. Field and C. J. Schofield, J. Chem. Soc., Chem. Commun., 1991, 1531 DOI: 10.1039/C39910001531
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