Base pairing of cytosine analogues with adenine and guanine in oligonucleotide duplexes: evidence for exchange between Watson–Crick and wobble base pairs using 1H NMR spectroscopy
Abstract
1 H NMR spectra of oligodeoxynucleotide duplexes incorporating N4-methoxycytosine and a related analogue paired with adenine and guanine demonstrate that Watson–Crick and wobble base pairs are in slow exchange on the chemical shift timescale.