The first ESR spin-trapping evidence for the formation of hydroxyl radical from the reaction of copper(II) complex with hydrogen peroxide in aqueous solution

Abstract

The formation of hydroxyl radical (OH·) during the reaction of copper(II) ion with hydrogen peroxide (H₂O₂) is determined by ESR spectroscopy using water-soluble spin-traps, 5,5-dimethyl-1-pyrroline N-oxide (DMPO, 1), α-(4-pyridyl-1-oxide)-N-tert-butylnitrone (POBN, 2) and sodium 3,5-dibromo-4-nitrosobenzenesulphonate (DBNBS, 3).