Communication. Cyclodextrin-modified micellar electrokinetic chromatography combined with semiconductor laser fluorimetry

Abstract

Two polycyclic aromatic compounds, 1-aminoanthracene and benzo[a]pyrene, have been separated by cyclodextrin-modified electrokinetic chromatography and detected by laser fluorimetry. For miniaturization of the fluorimetric system, a blue semiconductor laser (415 nm, 50 µW), i.e., the second-harmonic emission of a near-infrared semiconductor laser (830 nm, 40 mW), was used. The detection limit achieved for 1-aminoanthracene was of the order of 1 × 10^–8 mol dm^–3, the absolute detection limit being at the femtogram level. The detection limit achieved for benzo[a]pyrene was restricted to about 1 × 10^–6 mol dm^–3, owing to the discrepancy between the wavelength of the laser emission and that of the absorption maximum (390 nm).