Rapid and selective high-performance liquid chromatographic method for the determination of Michler's ketone in Methyl Violet 2B

Abstract

A high-performance liquid chromatographic method for the determination of ppm levels of Michler's ketone (MK) in Methyl Violet 2B (MV) is described. The direct determination of MK in a methanolic solution of MV is made possible by the presence of a spectral window between 300 and 400 nm in the spectrum of MV. This simple isocratic high-performance liquid chromatographic method gave a limit of detection for MK of 1 ppm.