Iodimetric method for the determination of mono- and disaccharides with vanadium(V) in perchloric acid

Abstract

Sodium (or ammonium) metavanadate in dilute perchloric acid was found to be a suitable reagent for the determination of fructose, mannose, galactose, arabinose, ribose, xylose, sucrose and maltose. A mixture containing the saccharide and an excess of the reagent was refluxed over a small flame for 40 min. The residual metavanadate was determined iodimetrically. The amount of saccharide was then determined by standardizing the metavanadate solution against either sodium thiosulphate solution or the respective saccharide solution of known strength; the latter procedure gave consistently better results.