Michael addition as the key step in the syntheses of sialyloligosaccharide–protein conjugates from N-acryloylated glycopyranosylamines

Abstract

Sialic acid and sialyloligosaccharides having terminal N-acryloyl functionality in their aglycone portions were efficiently conjugated to the lysine groups of proteins through Michael additions under mild basic conditions; the same N-acryloyl precursors were also polymerized with acrylamide to form serologically useful antigens.