Issue 10, 1990

Method for the determination of radioactivity in the C-2 carbon of glycine

Abstract

A method for the determination of the radioactivity of the C-2 carbon of glycine has been developed. The method is based on the degradation of glycine (135–137 °C for 50 s) to carbon dioxide and formaldehyde using chloramine T. The formaldehyde originated from the C-2 carbon of glycine was trapped with dimedone (5,5-dimethyl-1,3-cyclohexanedione) and the formaldehyde-dimedone complex extracted with toluene. The radioactivity of the toluene was a measure of the radioactivity in the C-2 carbon of glycine. Using (2-14C)glycine, the percentage recoveries ranged from 88 to 105%, whereas with (1-14C)glycine, these were <0.5%, suggesting that the label of the C-1 carbon did not interfere with the determination of radioactivity in the C-2 carbon of glycine. The method is simple, less time consuming than existing methods and a large number of samples can be handled at a time.

Article information

Article type
Paper

Analyst, 1990,115, 1309-1311

Method for the determination of radioactivity in the C-2 carbon of glycine

H. P. S. Makkar and P. J. Buttery, Analyst, 1990, 115, 1309 DOI: 10.1039/AN9901501309

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements