Continuous-flow solid-phase synthesis of a 74-peptide fragment analogue of human β-chorionic gonadotropin

Abstract

The linear dimer of a triacontaheptapeptide corresponding to the C-terminal of the β-subunit of human chorionic gonadotropin has been synthesized by the continuous-flow Fmoc-polyamide solid-phase method. The S-protected peptide was obtained in good yield after purification, suggesting that this synthesis procedure may be generally applicable for the assembly of large sequences. Potential problems with the method are discussed with reference to those experienced in this assembly.