Studies on the determination of selenium by hydride generation non-dispersive atomic fluorescence spectrometry using hydrobromic acid-based reaction matrices

Abstract

Selenium was determined by hydride generation coupled with non-dispersive atomic fluorescence detection, using hydrobromic acid-based aqueous matrices in both the pre-treatment stage and the primary hydride generation. Attention was focused on the interference problems arising from the presence of nitric acid, nitrite, chlorine and hypochlorites, and also on the capability of the system to recover selenate and selenomethionine. Determination of selenium in fish muscle, fish liver and sea water certified reference materials and in chlorine-treated water samples is reported.