High-performance liquid chromatographic technique for the determination of the polyether antibiotic lysocellin sodium

Abstract

A high-performance liquid chromatographic technique is described for the polyether antibiotic lysocellin sodium. The technique was developed because the methods currently used for polyether antibiotics are not applicable to lysocellin sodium. This is because lysocellin sodium either lacks a chromophore, does not react with vanillin or lacks the stability needed for derivatisation. The proposed technique involves the use of a UV-absorbing ion pair and is rapid and specific for lysocellin sodium. A method for the determination of lysocellin sodium in fermentation broth which uses the UV-absorbing ion-pair technique is discussed.