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Issue 5, 1989
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High-performance liquid chromatographic determination of bromazepam in human plasma

Abstract

An assay using high-performance liquid chromatography has been developed for the determination of bromazepam in plasma. After a single-step extraction from basified samples with dichloromethane, using decarboxyloflazepate as an internal standard, samples were analysed using a reversed-phase Nova Pak 5-µm column with a mobile phase of methanol-phosphate buffer (60 + 40) adjusted to pH 7.6. The drugs were detected at 239 nm and the limit of detection was found to be 3 µg l–1 for bromazepam. The method is simple, rapid and sensitive and permits bromazepam levels in clinical and pharmacokinetic studies to be monitored.

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Article type: Paper
DOI: 10.1039/AN9891400639
Analyst, 1989,114, 639-641

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    High-performance liquid chromatographic determination of bromazepam in human plasma

    A. Boukhabza, A. A. J. Lugnier, P. Kintz, A. Tracqui, P. Mangin and A. J. Chaumont, Analyst, 1989, 114, 639
    DOI: 10.1039/AN9891400639

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