Kinetic and chemometric studies of the determination of creatinine using the Jaffé reaction. Part I. Kinetics of the reaction: analytical conclusions

Abstract

A kinetic-spectrophotometric study of the Jaffé reaction was carried out and the kinetic behaviour, calibration step and interfering effect of albumin on creatinine standard solutions were studied. It was concluded that there is a variation in the kinetic behaviour of the system when higher concentrations of creatinine, picrate or sodium hydroxide are tested. The experimental conditions for quantifying creatinine must be chosen so that the kinetic behaviour is the same in the dynamic concentration range. Changes in the absorbance (ΔA)versus concentration equations were chosen as the most suitable for calibration graphs. It was also shown that creatinine results will have a proportional bias error if the interfering effect of albumin is not taken into consideration.