Terbium chelate labels for fluorescence immunoassays

Abstract

Bovine serum albumin was labelled with a terbium complex by means of a reagent prepared from diethylenetriaminepentaacetic acid or diethylenetriaminepentaphosphonic acid and p-aminosalicylic acid. The ternary mixed ligand complexes showed strong fluorescence and were stable in very dilute solution (10^–10M). The fluorescence of the conjugated products was compared with regard to their application in the fluoroimmunoassay of bovine serum albumin.