Peptide synthesis. Part 13. Feedback control in solid phase synthesis. Use of fluorenylmethoxycarbonyl amino acid 3,4-dihydro-4-oxo-1,2,3-benzotriazin-3-yl esters in a fully automated system

Abstract

A spectrometric technique is described for monitoring the course of acylation reactions by the title esters in solid phase peptide synthesis. The procedure has allowed construction of a fully automated peptide synthesizing system in which acylation and deprotection steps may be checked automatically for completion before proceeding to the next amino acid residue.