Selective disruption of 1H–1H dipolar connectivities within two-dimensional nuclear overhauser effect spectra by paramagnetic centres

Abstract

A new approach is presented, based on selective disruption of dipolar ¹H–¹H connectivities within two-dimensional nuclear Overhauser effect (2D NOE) spectra by paramagnetic centres, that allows detection of structural features in paramagnetic complexes. The interaction between Mn^II and adrenalin is considered. The binding sites within the ligand molecule are easily delineated; moreover, the range of exchange processes that can be handled with the 2D NOE approach is greatly amplified in comparison with methods based on measurement of nuclear paramagnetic rates.