Electrochemical regeneration of NAD+. A new evaluation of its actual yield

Abstract

Owing to its actual yield being better than 99.99%, i.e. a coenzyme turnover number > 10 000, the electrochemical oxidation of NADH must be considered as one of the best methods of regeneration of enzymatically active NAD⁺ in terms of efficiency. Owing to its intrinsic simplicity of execution it could be very useful in the development of selective oxidations involving dehydrogenases. The sole cause of deactivation of the coenzyme is the acid-catalysed hydrolysis of NADH, a reaction not related to the method of regeneration.