Combination of a new amide-precursor reagent and trimethylsilyl bromide deprotection for the Fmoc-based solid phase synthesis of human pancreastatin and one of its fragments (Fmoc = fluoren-9-ylmethoxycarbonyl)
Abstract
A 52- and a 29-residue peptide amide corresponding to human pancreastatin and one of its fragments were synthesized by the Fmoc-based solid phase techniques (Fmoc = fluoren-9-ylmethoxycarbonyl), and a new amide precursor reagent, 3-(α-Fmoc-amino-4-methoxybenzyl)-4-methoxyphenylpropionic acid, was employed in combination with thioanisole-mediated trimethylsilyl bromide deprotection; the synthetic peptides significantly inhibited protein output, but not juice flow or bicarbonate output from rat pancreas.