Formation of acyclic α- and γ-ketols and 12-oxophytodienoic acid from linolenic acid 13-hydroperoxide by a flax enzyme preparation. Evidence for a single enzyme leading to a common allene epoxide intermediate

Abstract

Experiments with [14-²H₂]linolenic acid confirm the requirement for a 13-hydroperoxide for 13-oxophytodienoic acid (12-oxoPDA) formation by a flax enzyme preparation; an allene epoxide intermediate is capable of explaining the formation of the acyclic α- and γ-ketols as well as 12-oxoPDA, suggesting the involvement of a single enzyme.