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Issue 7, 1988
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Amperometric determination of p-hydroxybenzoic acid esters using an oxygen electrode

Abstract

An enzyme method for the determination of p-hydroxybenzoic acid esters is described based on the indirect electrochemical monitoring of p-hydroxybenzoic acid via its reaction with oxygen in the presence of p-hydroxybenzoate hydroxylase. Derivative amperometric signals due to oxygen depletion enable a one-point kinetic analysis to be carried out. Esterase hydrolyses p-hydroxybenzoic acid esters to the free acid and p-hydroxybenzoate hydroxylase catalyses the reaction of p-hydroxybenzoic acid with NADPH to produce NADP+, which is accompanied by a stoicheiometric consumption of oxygen. The method has been successfully applied to the analysis of real soft drink samples.

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Article information


Analyst, 1988,113, 1035-1037
Article type
Paper

Amperometric determination of p-hydroxybenzoic acid esters using an oxygen electrode

T. Hamano, Y. Mitsuhashi, N. Aoki, S. Yamamoto and Y. Oji, Analyst, 1988, 113, 1035
DOI: 10.1039/AN9881301035

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