Determination of orotic acid (vitamin B13) in human serum and urine by differential-pulse polarography

Abstract

A differential-pulse polarographic method for the determination of orotic acid in urine and human serum is proposed. The effects of the amount of sample taken and the concentration of HClO₄ present were investigated. The detection limit was 1.54 µg ml^–1. The standard deviation of the determination of 15.4 µg ml^–1 of orotic acid in 5 ml of urine was 0.50 µg ml^–1 and that of the determination of 15.4 µg of orotic acid in 1 ml of human serum was 0.28 µg ml^–1.