In vitro production and biosynthesis of fomajorin D and S by Fomes annosus(Fr.) Cooke

Abstract

Incorporation of [1-¹³C]-, [2-¹³C]-, and [1,2-¹³C₂]-acetates and [2-¹³C]-mevalonate into fomajorin D supports its biosynthetic derivation from a humulene-type precursor via cyclization of trans, trans-farnesyl pyrophosphate. [1,2-¹³C₂]Acetate derived fomajorin D was not converted in vitro into fomajorin S. However, the stereochemistry of fomajorin S is proposed on the basis of its biosynthetic origin.