Generation of radicals from antioxidant-type molecules by polyunsaturated lipids

Abstract

A method for the formation of radicals from antioxidant-type compounds which makes use of the radicals generated during the autoxidation of polyunsaturated lipids is described. This simple system allows observation of radicals by e.s.r. spectroscopy as well as the study of radical exchange reactions in conditions similar to those encountered during lipid autoxidation and biological peroxidation reactions. Advantages and limitations are discussed. Applications presented include studies of the fate of antioxidants and the role of oxygen during lipid autoxidation, investigations of radical exchange reactions occurring in living cells, and of the role of oxidative reactions in the antipsoriatic drug action of anthralin.