Spectrophotometric determination of chloramphenicol using orthogonal polynomials

Abstract

A direct spectrophotometric method using combined orthogonal polynomials (P_W) for the determination of chloramphenicol in the presence of its alkali-induced degradation products is described. Mixtures of the intact drug with its degradation products were prepared and their absorbances were measured over the wavelength range 248–314 nm. The p_W coefficient calculated over this range at 6-nm intervals was found to be reproducible and independent of the degradation products. The proposed method proved to be applicable to monitoring chloramphenicol stability.