Methods for the oxidation of the cofactor, NADH, are discussed and the criteria for a successful mediator are set out. The kinetics of the system are analysed and linear free-energy relationships found for a number of different mediators. The variation of the reaction rate with pH is discussed. The successful oxidation of NADH using a modified electrode with Meldola's blue is described.
L. Gorton, J. Chem. Soc., Faraday Trans. 1, 1986, 82, 1245 DOI: 10.1039/F19868201245
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