Chemically modified electrodes for the electrocatalytic oxidation of nicotinamide coenzymes
Methods for the oxidation of the cofactor, NADH, are discussed and the criteria for a successful mediator are set out. The kinetics of the system are analysed and linear free-energy relationships found for a number of different mediators. The variation of the reaction rate with pH is discussed. The successful oxidation of NADH using a modified electrode with Meldola's blue is described.