Collaborative studies of methods for the detection of residues of monensin in chicken tissues

Abstract

Some published methods for the detection of residues of monensin in chicken tissues were examined. In some instances the limits of detection claimed by the authors could not be achieved. A method was evaluated in which the monensin was extracted from the tissues with methanol, partitioned into carbon tetrachloride and then purified on a silica gel column. Monensin was determined in the eluate from this column by TLC. Alternative procedures for detecting the monensin on the TLC plate by bioautography or by formation of a fluorescent derivative are given.