Amperometric enzyme electrode system for the flow injection analysis of glucose

Abstract

A flow injection system incorporating an amperometric enzyme electrode is described. Glucose oxidase immobilised on nylon mesh and held over a platinum electrode formed the basis of the electrode, which was incorporated in a three-electrode amperometric Stelte cell, modified for flow injection analysis. The flow and enzymatic reaction conditions have been optimised for maximum glucose response. The system exhibited good linearity (0.01 to 3.0 mM glucose), short response times (<45 s), good capacity (24 h with a continuous flow of 2.5 mM glucose) and long lifetimes (up to 4 months with storage at 4 °C). Data for the determination of glucose in foodstuffs with the enzyme electrode were similar to those obtained by the Yellow Springs Instrument glucose analyser and a soluble enzyme test kit (Boehringer Mannheim method).