Crown ethers and cryptands have been found to be selective complexing agents for a number of proteins, allowing their dissolution in non-aqueous solvents; by using 14C-radiolabelled 18-crown-6, an association constant of ca. 1.0 M–1 at 299 K for a cytochrome c–[1,2-14C2]-18-crown-6 complex was determined in methanol.
B. Odell and G. Earlam, J. Chem. Soc., Chem. Commun., 1985, 359 DOI: 10.1039/C39850000359
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