Jump to main content
Jump to site search
PLANNED MAINTENANCE Close the message box

Scheduled maintenance work on Wednesday 27th March 2019 from 11:00 AM to 1:00 PM (GMT).

During this time our website performance may be temporarily affected. We apologise for any inconvenience this might cause and thank you for your patience.


Issue 4, 1985
Previous Article Next Article

Gas chromatographic-mass spectrometric determination of muramic acid content and pyrolysis profiles for a group of gram-positive and gram-negative bacteria

Abstract

Two gas chromatographic-mass spectrometric methods, one based on chemical derivatisation and the other on analytical pyrolysis, were employed for the characterisation of a diverse group of bacteria. The muramic acid content of some Gram-positive and Gram-negative bacteria (including legionellae) was determined by aldononitrile acetate derivativisation and capillary GC-MS analysis. Muramic acid levels were generally higher in Gram-positive than in Gram-negative bacteria. When applied to the same set of bacteria, pyrolysis GC-MS and non-linear mapping were also able to distinguish Gram-negative from Gram-positive bacteria. The potential of GC-MS techniques for the direct chemical characterisation of bacteria is discussed.

Back to tab navigation

Article type: Paper
DOI: 10.1039/AN9851000381
Citation: Analyst, 1985,110, 381-385

  •   Request permissions

    Gas chromatographic-mass spectrometric determination of muramic acid content and pyrolysis profiles for a group of gram-positive and gram-negative bacteria

    L. W. Eudy, M. D. Walla, S. L. Morgan and A. Fox, Analyst, 1985, 110, 381
    DOI: 10.1039/AN9851000381

Search articles by author

Spotlight

Advertisements