Determination of B2 vitamers in serum of fish using high-performance liquid chromatography with fluorescence detection

Abstract

A sensitive method is described for the determination of riboflavin (RF), flavin mononucleotide (FMN) and flavin adenine dinucleotide (FAD) in serum of different fish using reversed-phase high-performance liquid chromatography (HPLC) with fluorimetric detection. Trichloroacetic acid was used to isolate B₂ vitamers from the serum, and an aliquot of this solution was analysed by HPLC with a Zorbax-NH₂ column with methanol-0.2 M phosphate buffer (1 + 9) as the mobile phase. The detection limits of RF, FMN and FAD in the serum were 4.89, 9.13 and 73.1 ng ml^–1, respectively.