Determination of cephalosporins utilising thin-layer chromatography with fluorescamine detection

Abstract

A thin-layer chromatographic procedure utilising fluorescamine detection is described for the quantitation of cephradine, cefaclor, cephalexin and cefadroxil. The repeatability, sensitivity and detectability of the method are compared with those for o-phthalaldehyde fluorescence emission and UV absorption measurements on the plate. The applicability of the fluorescamine method to the determination of these cephalosporins in serum, urine and pharmaceutical formulations is discussed.