Biosynthesis of the meroterpenoid, austin, by Aspergillus ustus: incorporation of 18O2, sodium [1-13C, 18O2]acetate, and [Me-13C, 2H3]methionine
Abstract
Mass spectral and 13C n.m.r. analyses of austin (1) produced by fermentation of Aspergillus ustus in the presence of 18O2 and [Me-13C,2H3]methionine showed that all labelled methionine-derived hydrogens were retained, determined that five of nine oxygens were introduced by aerobic oxidation, and elucidated the general mechanism of late biosynthetic stages.