Stereochemistry of hydride migration from C-24 to C-25 in the biosynthesis of priferasterol in Ochromonas malhamensis
Abstract
2 H-N.m.r. analysis of (2 S, 3 R)-2-([2H2]methyl)-[1,1,1,2-2H4]pentan-3-ol obtained from [2H22]poriferasterol, biosynthesized in the Chrysophyte Ochromonas malhamensis from C2H3CO2Na, shows that the hydride migration from C-24 to C-25 occurs on the si-face of the Δ24-precursor double bond.