Spectrophotometric determination of sulbactam by reaction with 1,2,4-triazole

Abstract

A simple and rapid spectrophotometric assay method for the determination of sulbactam has been developed. The method is based on the reaction with 2 M 1,2,4-triazole in solution (pH 10.0) for 15 min at 50 °C followed by measurement of ultraviolet absorbance (at 326 nm) of the reaction product. The method is quantitative for sulbactam concentrations down to 0.2 µg ml^–1, and specific to intact sulbactam without interference of penicillins, cephalosporins and degradation products of sulbactam.