Amperometric determination of glycerol and triglycerides using an oxygen electrode

Abstract

A new triglyceride or glycerol measurement system is described based on the indirect electrochemical monitoring of NADH via its reaction with oxygen by horseradish peroxidase. Derivative amperometric signals due to oxygen depletion provide for a one-point kinetic analysis. Lipase hydrolyses triglycerides to glycerol and glycerol dehydrogenase catalyses the reaction of glycerol with NAD to produce NADH. Serum measurements are demonstrated.