Biosynthesis of fomajorin D: incorporations of [1-13C]-, [2-13C]-, and [1,2-13C2]-acetates

Abstract

The ¹³C n.m.r. resonances of fomajorin D, a metabolite of Fomes annosus(Fr.) Cooke, have been assigned thus confirming the proposed structure (1); the labelling patterns of fomajorin D derived from [1-¹³C]-, [2-¹³C]-, and [1,2-¹³C₂]-acetates are in accord with its biosynthesis from farnesyl pyrophosphate in which cyclisation to a protoilludyl cation or its equivalent followed by oxidative cleavage of the appropriate bond would yield the metabolite.