Development and evaluation of a radioimmunoassay for the detection of amphetamine and related compounds in biological fluids
A radioimmunoassay has been developed for the detection of amphetamine and its analogues in blood and urine without any pre-treatment of the samples. It is based on a commercially available antiserum and a [125I] iodinated derivative of amphetamine.
The assay can detect low levels of amphetamine (less than 10 ng ml–1) in very small samples (50 µl) of blood and urine. It is cheap (3 pence per test), rapid, simple to perform and is specific for compounds closely related to amphetamine.
A high, positive correlation was obtained (r 0.93) when results of the analyses of urine samples from volunteers who had ingested amphetamine were compared with those produced by gas chromatography - mass spectrometry.
The assay has proved very useful for the detection of amphetamine and closely related compounds in biological fluids.