Application of 2H β-isotopic shifts in 13C n.m.r. spectra to biosynthetic studies. Incorporation of [1-13C, 2H3]acetate into O-methylasparvenone in Aspergillus parvulus

Abstract

Incorporation of [1-¹³C,²H₃]acetate into O-methylasparvenone (1) and analysis of the ²H β-isotope shifts in the resulting ¹³C n.m.r. spectrum indicate that one hydrogen is lost from the methyl derived from the acetate ‘starter’ unit; the magnitude of the observed ²H β-isotope shifts showed a marked dependence on the functionality of the reporter ¹³C nucleus and the stereospecificity of ²H incorporation.