Jump to main content
Jump to site search

Issue 1272, 1982
Previous Article Next Article

Determination of the non-starch polysaccharides in plant foods by gas-liquid chromatography of constituent sugars as alditol acetates

Abstract

A method is reported for the measurement of non-starch polysaccharides (NSP) from plant foods. NSP are the major components of “dietary fibre.” The polysaccharides are divided into cellulose and non-cellulosic material and the constituent sugars are determined by gas-liquid chromatography. Starch is removed, after gelatinisation, by incubation with hog pancreatic α-amylase together with pullulanase. The enzyme preparations are shown to be specific for the hydrolysis of α-1,4- and α-1,6-glucosidic bonds, and not to affect NSP. The starch-free material is then analysed by three separate but complementary procedures: (A) hydrolysis with 1 M sulphuric acid after solubilisation of cellulose with 12 M sulphuric acid; (B) hydrolysis with 1 M sulphuric acid; and (C) extraction with phosphate buffer at pH 7 and 100 °C, solubilisation of cellulose with 12 M sulphuric acid and then hydrolysis with 1 M sulphuric acid. Neutral sugars are measured by gas-liquid chromatography as alditol acetates and uronic acids by a colorimetric method. Starch made resistant to α-amylase digestion by food processing is identified by additional steps in procedure B, and measured as “resistant starch.” Procedure A gives total NSP and procedure B neutral non-cellulosic polysaccharides. A value for cellulose is obtained as the difference between glucose measured in procedures A and B. Procedure C gives NSP insoluble in phosphate buffer at pH 7. Soluble NSP is the difference between total NSP and insoluble NSP. Results for the NSP analysis of selected foods are given.

Back to tab navigation

Article type: Paper
DOI: 10.1039/AN9820700307
Citation: Analyst, 1982,107, 307-318
  •   Request permissions

    Determination of the non-starch polysaccharides in plant foods by gas-liquid chromatography of constituent sugars as alditol acetates

    H. Englyst, H. S. Wiggins and J. H. Cummings, Analyst, 1982, 107, 307
    DOI: 10.1039/AN9820700307

Search articles by author

Spotlight

Advertisements