Stability indicating assay for dipyrone. Part II. Separation and quantitative determination of dipyrone and its degradation products by high-performance liquid chromatography

Abstract

A rapid, sensitive and reproducible procedure for separation and quantification of dipyrone and three of the degradation products is proposed, using high-performance liquid chromatography on a reversed-phase column and ultraviolet detection. The method is about ten times more sensitive (detection limit 3–5 ng) than thin-layer chromatography and requires only a 12-min elution time. This method has been applied to the analysis of tablets and injections.