Issue 0, 1981

Photoaffinity labels for enzymes capable of binding glutathione or its disulphide

Abstract

The syntheses and photolyses of several glutathione analogues incorporating aryl azide moieties on the N-terminus or SH sites are described. Such labels include S-blocked glutathiones, N,S-blocked glutathiones and an N,N′-blocked glutathione disulphide. S-(4-Azidophenacyl)glutathione has been used to label covalently yeast glyoxalase I, beef liver glyoxalase II, and glutathione-S-transferase; N,S-bis(4-azidobenzoyl)glutathione has been used to label covalently formaldehyde dehydrogenases from Pseudomonas species and beef liver; N,N′-bis-(4-azidobenzoyl)glutathione disulphide has been successfully used to label covalently yeast glutathione reductase.

Article information

Article type
Paper

J. Chem. Soc., Perkin Trans. 1, 1981, 3029-3033

Photoaffinity labels for enzymes capable of binding glutathione or its disulphide

C. D'Silva, A. P. Seddon and K. T. Douglas, J. Chem. Soc., Perkin Trans. 1, 1981, 3029 DOI: 10.1039/P19810003029

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