Beta-induced fluorescence as a detection technique for liquid chromatography. Part 4.—Quenched beta-induced fluorescence

Abstract

The theory of beta-induced fluorescence is extended to include the quenching of fluorescence from excited solvent or dopant species by quenching agents. Expressions for the quenched beta-induced fluorescence (q.b.i.f.) response to efficient quenchers and the related signal-to-noise ratio are derived. Experimental results, obtained using samples containing polar groups (such as aldehydes) are compared with the theoretical predictions.