Spectrophotometric method for determination of chlorpheniramine maleate in pharmaceutical preparations in the presence of codeine phosphate and ephedrine hydrochloride

Abstract

A simple spectrophotometric method has been developed for the assay of chlorpheniramine maleate in raw materials and in pharmaceutical preparations. The method depends on the reaction of chlorpheniramine maleate with aniline in the presence of cyanogen bromide resulting in an intense yellow colour that has an absorption maximum at 482 nm at pH 7.0 and obeys Beer's law in the concentration range 50–175 µg ml^–1. The proposed method can be used for the determination of chlorpheniramine maleate in tablets, syrups and injections in the presence of codeine phosphate and ephedrine hydrochloride. The effect of pH and time on the formation of the derivative was also investigated.