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Issue 0, 1979
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Stereospecific hydrogen loss in the conversion of [2H7]isobutyrate to β-hydroxyisobutyrate in Pseudomonas putida. The stereochemistry of β-hydroxyisobutyrate dehydrogenase

Abstract

Ammonium [2H7]isobutyrate was converted by Pseudomonas putida(ATCC 21 244) into a mixture of (2S)β-hydroxy[3-2H2,2′-2H3]isobutyric acid (69%) and (2S,3S)-β-hydroxy[3-2H1,2′-2H3]isobutyric acid (31%). The latter is formed stereospecifically with loss of the 3-pro-R-hydrogen atom, probably via reversible formation of methylmalonic semialdehyde by the enzyme β-hydroxyisobutyrate dehydrogenase.

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Article type: Paper
DOI: 10.1039/P19790001404
J. Chem. Soc., Perkin Trans. 1, 1979, 1404-1406

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    Stereospecific hydrogen loss in the conversion of [2H7]isobutyrate to β-hydroxyisobutyrate in Pseudomonas putida. The stereochemistry of β-hydroxyisobutyrate dehydrogenase

    D. J. Aberhart and C. Hsu, J. Chem. Soc., Perkin Trans. 1, 1979, 1404
    DOI: 10.1039/P19790001404

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