Synthesis by conventional methods of bovine and ovine growth hormone-(125–133)-nonapeptides

Abstract

Two nonapeptides, Arg-Glu-Leu-Glu-Asp-Gly-Thr-Pro-Arg and Arg-Glu-Leu-Glu-Asp-Val-Thr-Pro-Arg, corresponding to the sequence 125–133 of bovine and ovine growth hormones (BGH and OGH) were prepared by conventional methods. A stepwise procedure was applied, active ester and dicyclohexylcarbodi-imide were used in the acylation reactions, and all the protected intermediates were isolated and characterized for homogeneity. Both peptides synthesized by solution method and OGH-(125–133) previously prepared by a solid-phase method showed very low biological activity in the hypophysectomized rat tibia epiphyseal width test.