Fluorimetric determination of acetohexamide in plasma and tablet formulations using 1-methylnicotinamide
Abstract
A sensitive method is described for the fluorimetric determination of acetohexamide in plasma or in tablets by means of its reaction with 1-methyl-nicotinamide, which is shown to be a useful reagent for the determination of ketonic compounds. The limit of detection is approximately 0.2 µg ml–1 and the relative standard deviation is 3.1% for 2 µg ml–1 in plasma. Acetoacetic acid usually does not interfere, but can be separated, if necessary, from acetohexamide by means of a washing technique. No interference is caused by the presence of insulin, other (non-ketonic) oral hypoglycaemic drugs, acetone or pyruvic or α-ketoglutaric acid.