Biosynthesis of 5,6-dimethylbenzimidazole from 6,7-dimethyl-10-[1′-14C]formylmethylisoalloxazine
Abstract
6,7-Dimethyl-10-[1′-14C]formylmethylisoalloxazine was added to growing cultures of Propionibacterium shermanii; the vitamin B12 subsequently isolated was found to be 14C-labelled exclusively in the C-2 carbon of the 5,6-dimethylbenzimidazole unit.