Cyclization of lycopene in vivo in a Flavobacterium species, R1519, suspended in deuterium oxide medium gives (2S,2′S)-[2,2′-2H2]zeaxanthin, thus demonstrating that the initial proton (deuteron) attack in the cyclization reaction is on the re,re face of the C-1,2 double bond.
G. Britton, W. J. S. Lockley, N. J. Patel, T. W. Goodwin and G. Englert, J. Chem. Soc., Chem. Commun., 1977, 655 DOI: 10.1039/C39770000655
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